Detection range: 96T 10 ng/L -320 ng/L Purpose of use: This kit is used to determine the midkine (MK) content in human serum, plasma and related liquid samples. Experimental principle This kit uses the double antibody sandwich method to determine the human midkine (MK) level in the specimen. The microporous plate was coated with purified human midkine (MK) antibody to prepare a solid phase antibody, and the midkine (MK) was sequentially added to the microcapsule of the coated monoclonal antibody, and then combined with the HRP-labeled midkine (MK) antibody. The antibody-antigen-enzyme-labeled antibody complex is formed, and after thorough washing, the substrate TMB is added for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with the midkine (MK) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the human midkine (MK) concentration in the sample was calculated from a standard curve. Kit Composition 1 30 times concentrated washing solution 20ml × 1 bottle 7 Stop solution 6ml × 1 bottle 2 Enzyme standard reagent 6ml × 1 bottle 8 standard product (640 ng / L) 0.5ml × 1 bottle 3 enzyme label coating plate 12 holes ×8 9 standard dilutions 1.5ml × 1 bottle 4 sample dilution 6ml × 1 bottle 10 instructions 1 part 5 color reagent A liquid 6ml × 1 bottle 11 sealing film 2 sheets 6 coloring agent B liquid 6ml × 1 / bottle 12 sealed bag 1
Featuring a simple, clean-lined silhouette and wood construction, this understated sideboard lends a midcentury modern touch to your dining room or parlour ensemble. Top it with stacked art books and contemporary statuettes to complete the mod look, or leave it bare for trays of cocktails and hors d'oeuvres when dinner guests arrive.
Easy to keep clean
Scratch proof
Shock proof
Resistant to friction
Heat and cooling resistant
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