European Red List Volatile Compound Analysis: The setup includes a DB-5.625 column (part number 122-5632) with dimensions of 30 meters by 0.25 mm internal diameter and a film thickness of 0.50 μm. For additional separation, a DB-624 column (part number 122-1334) is also used, which measures 30 meters by 0.25 mm with a 1.40 μm film thickness. The carrier gas is helium, flowing at a rate of 35 cm/sec, with the detector temperature set to 40°C. The column oven starts at 40°C for 2 minutes, then increases to 140°C at a rate of 12°C per minute. The injection is performed in split mode at 250°C with a split ratio of 1:50. The detector used is a Flame Ionization Detector (FID) operating at 300°C, with a nitrogen tail gas flow of 30 mL/min. A sample volume of 1 μL is injected using a headspace pure mixture. To ensure optimal performance, an advanced green septum (part number 5183-4759) is recommended. The liner is a straight type with an internal diameter of 1.5 mm (part number 18740-80200), and a splitter plate gasket (part number 18740-20885) is used for better sample introduction. This configuration ensures high sensitivity and accurate detection of volatile compounds in complex matrices.
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