European Red List Volatile Compound Analysis: The setup includes a DB-5.625 column (part number 122-5632), 30 m x 0.25 mm with a 0.50 μm film thickness, and a DB-624 column (part number 122-1334), 30 m x 0.25 mm with a 1.40 μm film. Helium is used as the carrier gas at a flow rate of 35 cm/sec. The detector temperature is set to 300°C, while the oven starts at 40°C for 2 minutes, then increases to 140°C at a rate of 12°C/min. The injection port is set to 250°C in split mode with a split ratio of 1:50. A flame ionization detector (FID) is used for detection. Nitrogen is used as tail gas at 30 mL/min. The sample volume is 1 μL, and a headspace pure mixture is recommended. For optimal performance, an advanced green septum (part number 5183-4759) is suggested. The liner is straight with a 1.5 mm inner diameter (part number 18740-80200), and a splitter plate gasket (part number 18740-20885) is used to ensure proper sealing and efficient separation. This configuration is ideal for accurate and reliable analysis of volatile compounds in complex samples.
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