Shanghai Haozhuang Instrument Co., Ltd. Haozhuang (LNB) brand biological safety cabinet is used to protect the operator, the laboratory environment, and the infectious experimental materials such as primary cultures, bacterial strains, and diagnostic specimens. The experimental materials are designed to avoid exposure to infectious aerosols and spills that may be generated during the above operations.
For the technical indicators of Shanghai Haozhuang Biological Safety Cabinet, please refer to the following indicators:
1. The velocity of position air entering the biosafety cabinet through the front opening is approximately 0.45m / s. Directional airflow at this speed is extremely susceptible to interference, including the airflow created by people approaching the biosafety cabinet, opening windows, adjusting the air supply system, and opening and closing doors.
Therefore, the most ideal is that the biological safety cabinet should be located in the place where people move, the flow of goods and may disrupt the airflow. 30cm of space should be left behind the safety cabinet and on each side as much as possible to take advantage of the maintenance of the safety cabinet. There should be a space of 30-35cm above the safety cabinet, in order to accurately measure the speed of air passing through the exhaust filter, and to facilitate the replacement of the exhaust filter.
2. If the operator's biological safety cabinet is used improperly, its protective effect may be greatly affected. Operators need to be careful to maintain the integrity of the airflow at the front opening when moving their arms into and out of the safety cabinet. The arms should slowly enter and exit the front opening vertically.
Put your hands and arms into the biological safety cabinet and wait for about a minute, so that the safety cabinet is adjusted and the air inside can "sweep" the surfaces of your hands and arms before you can start to process the items. Before starting the experiment, place all necessary items in the safety cabinet to minimize the number of times the arms enter and exit the front opening.
3. The air grille in front of the second-level biological safety cabinet should not be blocked by paper, instruments or other objects. Items placed in the safety cabinet should use 70% alcohol to eliminate surface contamination. Experiments can be carried out on a towel soaked with disinfectant to absorb any droplets that may be spilled. All items should be placed as close to the edge of the table as possible at the back of the table, and so that it will not block the alternate grille during operation. Equipment that can generate aerosols (such as mixers, centrifuges, etc.) should be placed close to the rear of the safety cabinet. Larger items such as biohazardous waste bags, plates containing waste straws, and suction filter bottles should be placed on one side of the safety cabinet. The experimental operation on the work surface should be carried out from the clean area to the contaminated area.
Autoclavable biohazardous waste bags and straw trays should not be placed outside the safety cabinet, otherwise both arms must frequently enter and exit the safety cabinet when using these items, which will interfere with the integrity of the safety cabinet air barrier Thus affecting the protection of people and objects.
4. Operation and maintenance The design of most biological safety cabinets allows work 24 hours a day. The researchers also found that continuous work helps control the level of dust and particles in the laboratory. Class A1 and A2 biological safety cabinets that exhaust air into the room or are connected to a dedicated exhaust pipe through a bushing interface can be closed when not in use. Others, such as Class B1 and Class B2 biosafety cabinets, are installed by rigid pipes, and air flow must be maintained to maintain the balance of room air. Before starting work and after finishing work, let the safety cabinet work for at least 5 minutes to complete the "purification" process, and it should be left for the time when the polluted air is sent out of the safety cabinet.
5. UV lamp is not needed in the biological safety cabinet. If you use an ultraviolet lamp, you should clean it every week to remove dust and dirt that may affect its sterilization effect. When the safety cabinet is re-certified, the intensity of ultraviolet light should be checked to ensure that there is an appropriate amount of light emission. When someone is in the room, the UV lamp must be turned off to protect the eyes and skin and avoid injury caused by accidental exposure.
6. Open flames should be avoided in the environment of almost no microorganisms formed in the biological safety cabinet. The use of open flames will have an impact on the airflow, and when handling volatile and flammable materials, it is also dangerous. When sterilizing the inoculation ring, a micro burner or electric furnace can be used instead of an open flame.
7. The laboratory rules on how to deal with spillage should be posted in the overflow laboratory. Everyone who uses the laboratory must read and understand these rules. Once the overflow of biological hazards occurs in the biological safety cabinet, it should be cleaned up immediately when the safety cabinet is in working condition. Use effective disinfectants and minimize the formation of aerosols during processing. All materials that come into contact with spilled items should be disinfected and / or autoclaved.
8. The evaluation of the protective effect of the safety cabinet should include the integrity of the safety cabinet, the leakage of the HEPA filter, the speed of the downward airflow, the speed of the positive airflow, the number of negative pressure / ventilation, the smoke pattern of the airflow, and the alarm and interlock The system is tested.
You can also choose to test leakage, light intensity, ultraviolet intensity, noise level and vibration. When conducting these tests, the inspectors must undergo special training and adopt special techniques and equipment.
9. Cleaning and disinfection As the remaining culture medium may cause the growth and reproduction of microorganisms, at the end of the experiment, all items in the biological safety cabinet including instruments and equipment should be clearly surface contaminated and removed from the safety cabinet. Before and after each use, the internal surface of the biological safety cabinet should be decontaminated. The work surface and inner wall should be wiped with disinfectant. The disinfectant used should be able to kill any microorganisms that may be found in the safety cabinet. At the end of each day's test, the work surface of the biosafety cabinet, the periphery, and the inside and outside of the glass should be wiped to remove surface contamination. When it is effective for the target organism, it can be disinfected with bleach solution or 70% alcohol. After using corrosive disinfectants such as bleach, it must be wiped again with sterile water. It is recommended to keep the safety cabinet running at all times. If you want to shut down, you should run for 5 minutes before shutting down to purify the gas inside.
10. Decontamination Before moving and replacing the filter, the biological safety cabinet must be decontaminated. The most common method is to use formaldehyde steam fumigation. Qualified professionals should remove the contamination of the biological safety cabinet.
11. Personal protective equipment should wear personal protective clothing when using biological safety cabinets. During the operation of the first and second level of biological safety, you can wear ordinary test clothes. The back-reinforced test isolation jacket with front reinforcement treatment has better protection effect, and should be used when performing the operations of the third and fourth level biological safety level (except the protective clothing laboratory). Gloves should be worn outside the gown, and sleeves with elastic bands can be worn to protect the researchers' wrists. Some operations may also require wearing masks and safety glasses.
12. The alarm can choose one of the two alarms to equip the biological safety cabinet. Window alarms can only be installed on safety cabinets with sliding windows. The alarm indicates that the operator moved the sliding window to an inappropriate position. When dealing with this kind of alarm, you only need to move the sliding window to an appropriate position. When the airflow alarm sounds, it indicates that the normal airflow mode of the safety cabinet has been disturbed, and the operator or item is immediately in a dangerous state. When the airflow alarm sounds, it should immediately stop working and notify the laboratory supervisor.
13. Select the correct type of biological safety cabinet, install and use it correctly, and certify every year at the same time, this is a complicated procedure. It is highly recommended to carry out the above work under the supervision of experts with good training and rich experience in biosafety operations. The expert should be very familiar with the relevant literature listed in the "References" section and have received training in all aspects of the biological safety cabinet. Operators should receive formal training in the operation and use of biological safety cabinets.
The company actively introduces new technologies, new processes, and uses branded raw materials and components to design, manufacture and produce a series of environmentally friendly and energy-saving laboratory instruments in accordance with industry standards. In the future, we will continuously improve the management level, scientific innovation, excellence and the optimization of the talent team to provide more high-quality new products for the majority of users.
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