1. Techniques of Liquid Chromatography for the Analysis of Aniline Substitutions in Organic Chemistry:
Effect of pH on the analysis of aniline substitutes (ORCH1)
Analysis conditions of aniline substitution in organic chemistry:
Aniline substitution analysis liquid chromatography column: ZORBAXStableBond-C18
Aniline substitution analysis chromatography column size: 4.6x150mm5µm
Part Number
Mobile phase
25mM phosphoric acid: methanol = 58: 42
Flow rate: 1mL / min
22 ° C
Changing the pH of the mobile phase can increase the efficiency of the column (improve the sensitivity), please see the figure for comparison:
1. p-methoxyaniline 2. m-benzylamine 3.3-amino-benzonitrile 4. p-chloroaniline 5. m-chloroaniline 6. o-chloroaniline
1.p-anisidine2.m-toluidine3.3-amino-benzonitrile4.p-chloroaniline5.m-chloroaniline6.o-chloroaniline
2. Characteristics of liquid chromatograph for analysis of aniline substitution in organic chemistry:
Liquid Chromatograph for Analysis of Aniline Substitutes in Organic Chemistry—Basic Technical Index of P600 High Pressure Constant Flow Pump:
Flow range: 0.001 ～ 9.999ml / min. Adjust the flow in 0.001ml / min steps.
Flow accuracy: RSD≤0.15% (1ml / min)
Flow accuracy: Ss≤2% (1ml / min water, 5 ～ 10MPa room temperature)
Maximum working pressure: 42MPa (0.001 ～ 9.999ml / min)
Pressure linearity and accuracy: display pressure error≤0.5MPa (0 ～ 42MPa)
Pressure pulsation: ≤0.2MPa (flow 1ml / min, pressure 5 ～ 10MPa)
The tightness of the pump: the pressure is 42MPa, the time is 10min, and the pressure drop is ≤0.5MPa.
Dimensions: 450mm × 300mm × 160mm (length × width × height)
Liquid Chromatograph for Analysis of Aniline Substitutes in Organic Chemistry-Basic Technical Specifications of UV600 Type UV Detector:
Wavelength range: 190 ～ 600nm
Spectral bandwidth: 8nm
Wavelength indication error: ≤2nm
Wavelength repeatability: ≤0.5nm
Baseline noise (peak-to-peak value): ≤5 × 10-5AU (mobile phase methanol, 254nm)
Baseline drift (peak-to-peak value): ≤5 × 10-4AU (mobile phase methanol, after stabilization, 254nm)
Minimum detection amount: 2 × 10-8g / ml (methanol solution of naphthalene)
Dimensions: 450mm × 300mm × 160mm (length × width × height)
The basic functions of liquid chromatograph-chromatographic workstation for the analysis of aniline substitution in organic chemistry are:
1. Six quantitative calculation methods: normalization method, modified normalization method, modified normalization method with scale factor, internal standard method, external standard method and index calculation method.
2. Calibration operation: It can realize the calibration using a variety of standard samples with different concentrations, and establish the sample concentration-area calibration curve.
3. Flexible peak identification and processing capabilities: You can identify peak chromatographic peak processing by setting peak processing parameters and time programs, or you can manually process it.
4. Chromatogram graphs, set quantitative calculation methods, peak processing parameters and peak identification tables can be saved in a file named by the user.
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