First, the general operation steps:
1). First filter the mobile phase, and select different filters according to your needs, generally organic and water. The commonly used pore sizes are 0.20um and 0.45um.
2). Ultrasonic degassing of the filtered mobile phase for 10-20 minutes.
3). Under normal circumstances, the instrument is first rinsed with methanol for 10-20 minutes, and then into the mobile phase for testing (if the mobile phase is buffered, it should be washed twice with distilled water for 10-20 minutes until the column is organic As far as the net is concerned).
4). Under normal circumstances, after the mobile phase is washed for 20-30 minutes, the instrument can be stabilized. The most important thing is that the instrument can be injected after the baseline is taken.
5). At the same time, the two needles are sampled, and the results are compared. After the ratio of the results is between 0.98 and 1.02, the sample can be formally tested.
6). After the sample is tested, the chromatograph and column should be cleaned and maintained. If the mobile phase is a buffer reagent, it should also be washed with distilled water for 10-20 minutes before it can be protected with an organic phase. Otherwise, the column is damaged.
7). When shutting down, turn off the computer first, then turn off the liquid chromatograph.
8). Fill in the registration form and sign it by the person in charge.
Second, note:
1). The mobile phase requires chromatographic purity and water is 20M deionized water. The mobile phase after degassing should be carefully shaken to avoid air bubbles.
2). The column is very fragile. For the first time, do not let the liquid pass through the column.
3). All liquid passing through the column requires strict filtration.
4). The pressure should not be too large, it is best not to exceed 150kgf/cm2.
5). Because the buffer reagent will crystallize in the presence of organic solvent and damage the column, each time the organic phase change mobile phase or mobile phase change organic phase needs to be washed with distilled water.
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