Detection of soluble protein: prepare serum and plasma specimens according to the product instructions. Because some samples are sensitive to temperature and easily lose their activity, they should be separated and packaged as soon as possible (generally 300 ～ 500ul / tube). Most specimen collection and experiments should be stored on the same day At 2 ～ 8 ℃. If it cannot be measured immediately, it should be stored at -70 ℃. The storage time of specimens is generally not too long to avoid repeated freezing and thawing. Balance the specimen to room temperature before use. It is strictly forbidden to use a water bath to melt the specimen. Specimens or small agglomerates may appear in specimens that have been frozen or stored for too long, and should be centrifuged or filtered (0.45um filter membrane) before the experiment. Filtration of specimens with small volume is not recommended. All specimens should be collected in sterile tubes and operated under sterile / semi-sterile conditions to prevent contamination.
Serum: Agglutinate the blood at room temperature for 10-15 minutes, centrifuge at 1500g for 20-30 minutes, and carefully aspirate the serum.
Plasma: Select the anticoagulant according to the instructions in the kit. When collecting blood, gently shake the test tube to mix the anticoagulant with the blood. Store the blood at room temperature for 10-15 minutes, centrifuge at 1500g for 15-20 minutes, and carefully aspirate the upper plasma.
Urine: Collect urine in a sterile container. Centrifuge at 1500g for 10-15 minutes or filter (0.45um filter membrane) to remove impurities. Note: The regression coefficients for urine specimen experiments may change.
Cell culture supernatant: centrifuge at 1500g for 10 to 15 minutes to remove cells and cell debris. If a conventional typical medium is used, two standard curves should be prepared, one to prepare the standard using the standard dilution and / or experiment buffer, and the other to prepare the standard using cell culture medium. If the variation range of the two standard curves is within 10%, the experimental results can be analyzed with either curve.
Detection of cell-related and intracellular proteins: Cell-related proteins and other intracellular proteins need to be processed to release the target protein before the ELISA experiment.
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