The standard of ideal liquid chromatography column
1. Good peak shape and good column efficiency
——For any analyte (acidic, alkaline and neutral)
Poor peak shape will result in:
1. The method development is more difficult and the process is prolonged
2. The analysis results are inconsistent
3. Insufficient chromatographic resolution
4. Reduced detection sensitivity
Consequences of poor peak shape:
Impact of poor peak shape on integration accuracy:
The adverse effect of poor peak shape on peak resolution:
The effect of poor peak shape on detection sensitivity:
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2. Long column life
——Good performance at different pH and column temperature
3. Chromatographic separation can be easily amplified
——From the scale of analysis to the scale of preparation directly
Fourth, the separation performance is highly reproducible
——This is required between columns and columns, between different batches of fillers and between different particle size fillers
5. The traceability of the quality of the chromatographic column product and whether the chromatographic column production process complies with the latest GMP regulations
Six, the price is moderate
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