Ca2 + and H + play an important role in pollen tube growth, directional elongation and morphogenesis. The elongation of differentiated cells in the pollen tube requires the presence of Ca2 + and H + concentration gradients. Because lily is not easy to build a stable transgenic system, it is not suitable as a model plant for molecular genetic research. Tobacco transgene and cell biology are more in-depth research, and its pollen tubes are also easy to obtain, so it is more suitable as a model for pollen tube research. material. Researchers such as Portuguese scientists Feijó and Michard used tobacco as the experimental material and transiently expressed pHluorin and yellow Cameleon protein as probes for the ratio imaging analysis of intracellular H + and Ca2 +, respectively. Obtained, and the Fourier decomposition method and continuous wavelet analysis method were used to analyze the ion concentration gradient, ion current and growth rate during pollen tube elongation. The results showed that there was a gradient of 0.4pH unit at the tip of the tobacco pollen tube, and there was a sub-tip alkalized region on the pollen tube. The extracellular proton flow oscillation is about 10 ～ 40 pmol·cm-2·s-1, there are one or two peaks in intracellular and intracellular H + oscillations, and there is a 0.2 ～ 1.0uM ion concentration gradient at the tip of the pollen tube. The oscillation period is 1 to 4 minutes, and the extracellular Ca2 + flow oscillation is about 2 to 50 pmol · cm-2 · s-1. Confocal and broad-spectrum microscopic observations showed that the patterns and shapes of H + and Ca2 + in pollen tube cells were different. This research uses non-damage micro-measurement technology and fluorescent indicator protein to improve the research accuracy of pollen tubes H + and Ca2 +. It is an example of combining molecular biology methods and physiological detection methods to study pollen tubes. Keywords: Pollen tube, Calcium signaling, Proton signaling, Cell polarization Reference: Erwan Michard. Et al. Sexual Plant Reproduction, 2008, 21: 169-181
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