Hanks Balanced Salt Solution (5× HBSS, with Phenol Red)
Hanks Balanced Salt Solution (HBSS) is a widely used buffer solution designed to mimic the physiological environment necessary for cell survival and metabolic activity in vitro. It maintains osmotic pressure, pH balance, and provides essential ions, glucose, and other nutrients required for cellular functions. HBSS typically contains sodium chloride, potassium chloride, sodium phosphate, sodium bicarbonate, glucose, calcium, magnesium, and phenol red as a pH indicator. Some formulations may exclude calcium and magnesium or contain HEPES to stabilize pH.
HBSS serves as a base for preparing complete cell culture media and is often used to dilute concentrated amino acid or vitamin solutions. Variants such as Hank’s BSS, Dulbecco’s PBS, EBSS, and PBS are commonly used in different experimental settings. The 5× HBSS formulation with phenol red is particularly useful for cell washing, tissue rinsing, and preparing cell dissociation reagents like trypsin-EDTA.
This product is supplied as a 5× concentrate and must be diluted to 1× before use. For general applications, it can be mixed with sterile water or deionized water in a 1:4 ratio. When working with cells, ensure aseptic conditions to prevent contamination. Due to its high salt content, slight precipitation may occur; if this happens, warm the solution gently to dissolve. If not soluble, discard the solution.
HBSS is filtered sterilized, has low endotoxin levels, and is stored at 4°C. The shelf life is 3 months. Always wear lab Gloves and a lab coat when handling the solution to ensure safety.
This product is ideal for a variety of cell culture and laboratory procedures, providing a reliable and consistent buffer for in vitro experiments.
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